TABLE 2.

Gene primer sequence, amplicon size, and source for eight virulence factors tested in E. coli

Gene	Primer namea	Primer sequence (5′→3′)	Length (bp)	E. coli positive control	Reference(s)
eae	eae-F	ATC TTC TGC GTA CTG CGT TCA	790	STJ348	5
	eae-R	CAT TAT GGA ACG GCA GAG GT		O157:H7
estA	STa-F	TCC CCT CTT TTA GTC AGT CAA CTG	171	P97-2554B	28, 37
	STa-R	GCA CAG GCA GGA TTA CAA CAA AGT		O149:K91
estB	STa-F	GCA ATA AGG TTG AGG TGA T	377	P97-2554B	23
	STb-R	GCC TGC AGT GAG AAA TGG AC		O149:K91
stx1	VT1-F	TTA GAC TTC TCG ACT GCA AAG	530	STJ348	44
	VT1-R	TGT TGT ACG AAA TCC CCT CTG		O157:H7
stx2	VT2-F	CTA TAT CTG CGC CGG GTC TG	327	STJ348	44
	VT2-R	AGA CGA AGA TGG TCA AAA CG		O157:H7
fedA1	F18-F	GTG AAA AGA CTA GTG TTT ATT TC	510	P88-1199	21
	F18-R	CTT GTA AGT AAC CGC GTA AGC		O139:K82
aidA-1	AIDA1-F	ACA GTA TCA TAT GGA GCC A	585	2787	6, 28
	AIDA1-R	TGT GCG CCA GAA CTA TTA		2787
elt	LT-F	TTA CGG CGT TAC TAT CCT CTC TA	275	P97-2554B	18
	LT-R	GGT CTC GGT CAG ATA TGT GAT TC		O149:K91

^aF, forward; R, reverse.