. 2009 Jan 5;75(5):1402–1409. doi: 10.1128/AEM.01899-08

TABLE 1.

Primer nucleotide sequences used to PCR amplify 10 viral groups

Human viruses	Primer	Sequence (5′-3′)^b	Sensitivity (no. of targets)	Amplicon size (bp)
Adenoviruses	AV-A1	GCC GCA GTG GTC TTA CAT GCA CAT C	100	300
	AV-A2	CAG CAC GCC GCG GAT GTC AAA GT
	AV-B1^a	GCC ACC GAG ACG TAC TTC AGC CTG		143
	AV-B2^a	TTG TAC GAG TAC GCG GTA TCC TCG CGG TC
Enteroviruses	JP UP	TTA AAA CAG CCT GTG GGT TG	100	600
	ENT DOWN	ACC GGA TGG CCA ATC
	ENT UP^a	CCT CCG CCC CTG AAT G		154
	JP DOWN^a	ATT GTC ACC ATA AGC GAC C
Hepatitis B viruses	HBS-1	ATC AGG ATT CCT AGG ACC C	10,000	310
	HBS-R1	AGG ACA AAC GGG CAA CAA C
	HBS-11^a	GCG GGG TTT TTC TTG TTG AC		241
	HBS-R11^a	GAA CCA ACA AGA AGA TGA GGC
Herpesviruses	FP1	GAY TTY GCI AGY YTI TAY CC	10	800
	FP2	TCC TGG ACA AGC AGC ARI YSG CIM TIA A
	RP1	GTC TTG CTC ACC AGI TCI ACI CCY TT
	FP3^a	TGT AAC TCG GTG TAY GGI TTY ACI GGI GT		215-235
	RP2^a	CAC AGA GTC CGT RTC ICC RTA IAT
Morbilliviruses	Up	ATG TTT ATG ATC ACA GCG GT	100	429
	Down	ATT GGG TTG CAC CAC TTG TC
Noroviruses	P290	GAT TAC TCC AAG TGG GAC TCC AC	10,000	219
	P289	TGA CAA TGT AAT CAT CAC CAT A
Papillomaviruses	FAP59	TAA CWG TIG GIC AYC CWT ATT	10,000	478
	FAP64	CCW ATA TCW VHC ATI TCI CCA TC
Picobirnaviruses (genotype I)	PicoB25	TGG TGT GGA TGT TTC	1	200
	PicoB43	ART GYT GGT CGA ACT T
Reoviruses	L1.rv5	GCA TCC ATT GTA AAT GAC GAG TCT G	100	416
	L1.rv6	CTT GAG ATT AGC TCT AGC ATC TTC TG
	L1.rv7^a	GCT AGG CCG ATA TCG GGA ATG CAG		344
	L1.rv8^a	GTC TCA CTA TTC ACC TTA CCA GCA G
Rotaviruses (group A)	RV1	GTC ACA TCA TAC AAT TCT AAT CTA AG	1,000	1,059
	RV2	CTT TAA AAG AGA GAA TTT CCG TCT G
	RV3^a	TGT ATG GTA TTG AAT ATA CCA C		346
	RV4^a	ACT GAT CCT GTT GGC CAW CC

Internal primer set for a nested PCR.

H equals A/C/T, M equals A/C, R equals G/A, S equals G/C, V equals G/C/A, W equals A/T, and Y equals T/G. Note that inosine (I) has base-pairing bias for C, A, G, and then T.