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. 2008 Dec 29;75(5):1331–1338. doi: 10.1128/AEM.01914-08

FIG. 2.

FIG. 2.

(A) Phenotypic appearance of wild-type smooth and rough M. abscessus mutant strains. (B) Southern blot analysis of the wild-type strain (lane 1), the single crossover (upstream from the kanamycin cassette) (lane 2), and the allelic-exchange mutant (lane 3) in M. abscessus using the upstream part of mmpL4b as a probe. (C) TLC analysis of the crude lipid fractions of the wild-type strain, (lane 1), a smooth kanamycin transformant (lane 2), an allelic-exchange mutant (lane 3), and the single crossover (upstream from the kanamycin cassette, lane 4) mutants of M. abscessus; TLC analyses of M. smegmatis wild type (lane 5) and mmpL4b mutant (lane 6) are also shown. TLC was performed using chloroform-methanol (9:1 [vol:vol]) as the solvent system and developed with iodine (250 μg each deposit).