TABLE 1.
Plasmids used in this study
| Plasmid | Featuresa | Source or reference |
|---|---|---|
| pBSK | Cloning vector; Ampr | Stratagene |
| pBSK-ΔmmpL4b | pBSK containing upstream and downstream regions of the mmpL4b gene | This study |
| pBSK-ΔmmpL4b::Km | pBSK containing the allelic-exchange substrate | This study |
| pPR27 | Mycobacterial replicating shuttle vector, containing the sacB gene and the Gmr cassette | 21 |
| pPR27-ΔmmpL4b::Km | pPR27 containing the allelic-exchange substrate | This study |
| pJSC347 | Vector containing PacI site and λ-cos | 33 |
| pJSC347-ΔmmpL4b::Km | pJSC347 containing the allelic-exchange substrate | This study |
| pJV53 | chec9 genes gp60_61 under the control of the acetamidase promoter | 44 |
| pJV53-zeo | pJV53, which the Kmr cassette replaced with a Zeor cassette | This study |
| pLYG204.Zeo | Plasmid encoding a Zeor cassette | 14 |
| pUC4K | Plasmid encoding a Kmr cassette | Pharmacia Biotech |
a
Ampr, ampicillin resistance; Gmr, gentamicin resistance; Kmr, kanamycin resistance; Zeor, zeocin resistance.