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Comparative counterselection efficiency of the ts-sacB system in M. smegmatis mc2155 and M. abscessus CIP104536T strainsa
| Transformant | CFU/ml in:
|
Frequency of counterselection (SD) | |
|---|---|---|---|
| LB (30°C) | LB + 10% sucrose (39°C) | ||
| M. smegmatis (pPR27-ΔmmpL4b) | 2 × 106 | 130 | 6 × 10−5 (9.8 × 10−6) |
| M. abscessus (pPR27-ΔmmpL4b) | 5 × 107 | 2 × 107 | 0.4 (0.06) |
Experiments were performed five times.
