TABLE 5.
Summary of the efficiencies of the ts-sacB, mycobacteriophage, and recombineering genetic systems examined in this studya
| Strain | Efficiency (mean %)
|
|||||
|---|---|---|---|---|---|---|
| ts-sacB
|
Mycobacteriophage
|
Recombineering
|
||||
| Rough clones | Allelic-exchange mutants | Rough clones | Allelic-exchange mutants | Rough clones | Allelic-exchange mutants | |
| M. smegmatis | 100 | 100 | 100 | 100 | 100 | 100 |
| M. abscessus | 0 | 0 | ∼6 | 0 | ∼15 | ∼7 |
a
The mean percentages of rough clones and allelic-exchange mutants expressed of all kanamycin-resistant clones are given.