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. 2008 Dec 29;191(5):1404–1413. doi: 10.1128/JB.01497-08

TABLE 1.

Bacterial strains and plasmids

B. subtilis strain or plasmid Relevant genotype Source or reference
Strains
    168 trpC2 Laboratory stock
    3728 trpC2 Ωneo3427 ΔmreB 20
    2505 trpC2 Ω(mbl::spc) 33
    2536 trpC2 Ω(mreBH::cat) 7
    KA trpC2 ΩamyE::PsigI-lacZ cat Kei Asai, unpublished
    4261 trpC2 Δmbl::cat This study
    4262 trpC2 ΔmreBH::erm This study
    4263 trpC2 ΔrsgI::neo This study
    4264 trpC2 ΔrsgI::(neo::spc) This study
    4265 trpC2 ΔsigI rsgI::neo This study
    4266 trpC2 Ω(mbl::spc) ΔrsgI::neo This study
    4267 trpC2 Ωneo3427 ΔmreB ΔrsgI::(neo::spc) This study
    4268 trpC2 ΔmreBH::erm ΔrsgI::neo This study
    4269 trpC2 Ω(mbl::spc) ΩamyE::(lacI PspacHY-sigI cat) This study
    4270 trpC2 Ω(mbl::spc) ΔsigI rsgI::neo ΩamyE::(lacI PspacHY-sigI cat) This study
    4271 trpC2 Ω(mbl::spc) ΩamyE::(lacI PspacHY-rsgI cat) This study
    4272 trpC2 Ω3427::neo ΔmreB ΩamyE::(lacI PspacHY-rsgI cat) This study
    4273 trpC2 ΔmreBH::erm ΩamyE::(lacI PspacHY-rsgI cat) This study
    4274 trpC2 Ωneo3427 ΔmreB Δmbl::cat ΔrsgI::(neo::spc) This study
    4275 trpC2 Ωneo3427 ΔmreB ΔmreBH::erm ΔrsgI::(neo::spc) This study
    4276 trpC2 Δmbl::cat ΔmreBH::erm ΔrsgI::(neo::spc) This study
    4277 trpC2 Ωneo3427 ΔmreB Δmbl::cat ΔmreBH::erm Ω(neo::spcrsgI This study
    4278 trpC2 Ω(mbl::spc) ΩamyE::PsigI-lacZ cat This study
    4279 trpC2 ΔrsgI::neo ΩamyE::PsigI-lacZ cat This study
Plasmids
    pMarB bla erm Pctc-Himar1 kan (TnYLB-1) 38
    pPL82 bla amyE::(lacI PspacHYcat) 47
    pBEST501 bla neo 31
    pVK71 bla neo::spc 8
    pMUTIN4 bla erm Pspac-lacZ lacI 56
    pLOSS* bla spc Pspac-mcs PdivIVA-lacZ lacI reppLS20 (GA→CC) 9
    pCotC-GFP bla cat PcotC-cotC-gfp 58
    pSG5916 pLOSS* containing sigI This study
    pSG5914 bla amyE::(lacI PspacHY- sigI cat) This study
    pSG5915 bla amyE::(lacI PspacHY-rsgI cat) This study