TABLE 2.
Oligonucleotides
| Oligonucleotide | Sequence (5′-3′)a | Restriction enzyme |
|---|---|---|
| rsgI-A | CATGATGTCGACGAGTGCAGCACCCCTTTAAG | SalI |
| rsgI-B | GGCGATATGACACTTGC | |
| rsgI-C | GATGACGGATCCCGGCGAATAAAGACCTG | BamHI |
| rsgI-D | CGTGGCGAAATGGAATC | |
| sigI-E | CTGACTGTCGACCTCAGTTCCTCCCTATAACTACTC | SalI |
| sigI-F | CAATAGATGCGCCAAGCAC | |
| sigI-fw | CCGTGTAAGCTTCCTTTGCGAATCCCTATC | HindIII |
| sigI-rev | GATACAGCATGCCATGCGTGTTTCACTCGGAAG | SphI |
| rsgI-fw | CAGCGTAAGCTTCAGCTTGAACAGCTTGTC | HindIII |
| rsgI-rev | GTAGCTGCATGCGGTACAGCAAGCACAATCAC | SphI |
| sigI-prom | GCGACTGGCTAGCGGCGTAATCTGAAAGACTCTGC | NheI |
| mbl-A | GCTCACTCTAGACCGAGGTCAATACCAATATCC | XbaI |
| mbl-B | GTGATGAAGCGTCCTATG | |
| mbl-C | CTGAGCGAATTCCGCAAACTAAGCTGATTTCAC | EcoRI |
| mbl-D | CCTATATGGCCTGGAAGAC | |
| mbl-fw | CTCGAGGATCCACCTGGCATTGCCTTCTTG | BamHI |
| mbl-rev | CATACTGAATTCCATGACACCTGTGCCCGATG | EcoRI |
| mreBH-A | CGTCAGTCTAGAGTTTTCACCCAATCTCTATCC | XbaI |
| mreBH-B | GTTCTCGCCTGCTCATTG | |
| mreBH-C | CGACTGGAATTCGCACAGGCCGTTCTTTAG | EcoRI |
| mreBH-D | GACCAGCATGTCTTACC | |
| mreBH-fw | TCTTTACCGACGCACACC | |
| mreBH-rev | CGATCGCTACTCTTCTTCC |
a
Restriction sites are underlined.