FIG. 3.

(A) Northern analysis of the sarZ and mgrA transcripts in the wild type (Wt), various isogenic mutants, and a single-copy complemented strain of the mgrA mutant at exponential phase (OD₆₀₀, ∼0.7) of growth. DNA fragments (500 bp and 550 bp) containing sarZ and mgrA genes, respectively, were used for hybridization. cpsmgrA indicates the complementation in single copy of the mgrA gene on the lipase locus (geh) of the mgrA mutant. (B) Northern analysis with a 500-bp sarZ DNA probe of the mgrA mutants and complemented (cpm) strains from different S. aureus strains, as indicated. The region of 23S rRNA of the ethidium bromide-stained gel used for blotting is also shown as a loading control in both panels.