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. 2008 Dec 19;191(5):1656–1665. doi: 10.1128/JB.01555-08

FIG. 7.

FIG. 7.

DNA binding activity of SarZ protein. Autoradiograms of 8.0% polyacrylamide gels showing the binding of SarZ protein to 287-bp and 250-bp promoter fragments (1 ng each or 5 and 6 fM, respectively) of sarS (A) and sspA (B), respectively. In panel A, lanes 1 to 5 correspond to 0 ng, 100 ng, 200 ng, 300 ng, and 500 ng, and lanes 6 to 8 correspond to 1.0 μg of purified SarZ protein. In panel B, lanes 1 to 5 correspond to 0 ng, 25 ng, 50 ng, 100 ng, and 200 ng, and lanes 6 to 8 correspond to 300 ng of purified SarZ protein. The mobilities of the bands were noted in the presence of increasing amounts of SarZ protein. A 50-fold excess (molar ratio) of specific unlabeled competitor DNA of the respective promoter fragments was used for competition in lanes 7 in both panels, whereas a 50-fold molar excess of a nonspecific competitor DNA (a 185-bp internal fragment of the sarX ORF region) is shown in lane 8 of each panel.