TABLE 1.

Genes and proteins of VSH-1 and B. hyodysenteriae B204 identified in this and previous studies^a

Gene(s)	ORF in B204 draft genome	Protein identity	Identification basis	Closest protein match (GenBank accession no.) and E value
VSH-1
hvp45 to hvp24	02481 to 02474	Head proteins	Protein sequencing	None
hvp53 to hvp28	02472 to 02467	Tail proteins	Protein sequencing	None
lys/hol	02465/02463	Endolysin/holin lysis proteins	Enzyme activity/predicted properties	Salmonella phage ε15 endolysin (AAO06088); E = 5 × 10−29
hvp31 to hvp37	02494 to 02497	Tail proteins	Protein sequencing	None
B. hyodysenteriae B204b
1	02501	AdSS	Conserved domains	Thermosinus carboxydivorans adenylosuccinate synthase (ZP_01666816.1); E = 10−146
2	02498	Unknown		Paramecium TPR protein (XP_001427675); E = 7 × 10−50
3	02493	Mcp	Conserved domains	B. hyodysenteriae methyl-accepting chemosensory protein (AAAX81982); E = 10−92
4	02491	Adh	Conserved domains	Caldicellulosiruptor saccharolyticus Fe-alcohol dehydrogenase (YP_001179521); E = 10−116
5	02490	Unknown		Tetrahymena TPR protein (XP_001026739); E = 10−22
6	02487	Trep		Treponema denticola unknown function protein (NP_972591); E = 8 × 10−34
7	02485	AA transport	Conserved domains	Fusobacterium nucleatum Ser/Thr transporter (NP_604045); E = 10−105
8	02484	Fe-S oxidoreductase	Conserved domains	Clostridium kluyveri Fe-S oxidoreductase (YP_001393555); E = 10−39
9	02461	Mcp	Conserved domains	B. hyodysenteriae methyl-accepting chemosensory protein (AAP58978); E = 4 × 10−94
10	02460	Mcp	Conserved domains	B. hyodysenteriae methyl-accepting chemosensory protein (AAP58978); E = 3 × 10−112
11	01437	NhaC	Conserved domains	Clostridium leptum Na+/H+ antiporter (ZP_02079635); E = 8 × 10−101
12	01435c	Unknown	Conserved domains	Ruminococcus gnavus unknown function protein (ZP_02040322); E = 3 × 10−58
13	01433c	Adh	Conserved domains	B. hyodysenteriae WA-1 alcohol dehydrogenase (ABS12704); E = 0.0

^aDescribed previously by Matson et al. (15). ORF number designations in column 2 refer to positions in the B. hyodysenteriae B204 draft genome. Closest protein matches in column 5 are all hypothetical proteins.

^bB. hyodysenteriae B204 genes (bacterial gene homologs) are labeled, for convenience, according to their positions in Fig. 1.

^cORFs 01433 and 01435 have adjacent gene homologs on the Ruminococcus gnavus genome map.