TABLE 3.

Summary of knockouts of H. volcanii acetylation-deacetylation genes

Gene	Status	Source
Single knockout
sir2	Viable	No selectable marker was used
hdaI	Essential	Knockout was obtained only in the presence of an extrachromosomal copy
pat1	Viable	Knockout was obtained using a selectable marker
pat2	Viable	Knockout was obtained using a selectable marker
elp3	Viable	Knockout was obtained using a selectable marker
Double knockout
pat1 pat2	Viable	Knockout was obtained using a selectable marker for both genes
pat1 elp3	Viable	Knockout was obtained using a selectable marker for both genes
pat2 elp3	Synthetic lethal	Knockout could not be obtained