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MfpAMt inhibits E. coli DNA gyrase supercoiling activity with an IC50 of 3 μM. Supercoiling assays were performed with 400 ng of relaxed pBR322 as the substrate (pBR) and 1 U of E. coli DNA gyrase with increasing concentrations of MfpAMt (μM) as indicated. R, Relaxed pBR322; S, supercoiled pBR322.
