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MfpAMt does not protect against E. coli gyrase inhibition by quinolones even in the presence of KGlu. (A) Comparison of inhibition by ciprofloxacin of pBR322 (pBR) supercoiling by E. coli gyrase in the presence of QnrB4 or MfpAMt at the same concentration (0.5 μM). R, relaxed pBR322; S, supercoiled pBR322. (B) Similar experiments with 100 mM KGlu in the assay buffer.
