TABLE 1.

Effects of MfpA_Mt and QnrB4 on DNA gyrases

Type of effect	Type II topoisomerase	Effect of:
		MfpAMt	QnrB4
Direct effect on catalysis	M. tuberculosis DNA gyrase	Inhibition of supercoiling activity (IC50, 2 μM)	No effect until 20 μM
	E. coli DNA gyrase	Inhibition of supercoiling activity (IC50, 3 μM)	No effect until 30 μM
Protection from fluoroquinolone inhibition	M. tuberculosis DNA gyrase	No protection, no modification of ciprofloxacin or moxifloxacin IC50	No protection, no modification of ciprofloxacin IC50
	E. coli DNA gyrase	No protection, no modification of ciprofloxacin IC50	Concentration-dependent protective effect, increase of ciprofloxacin and moxifloxacin IC50s in the presence of QnrB4