TABLE 2.
Relative expression levels of glmS and of genes involved in the competence pathway in a yvcJ mutant strain from transcriptome analysis
| Gene | Expression level | Description |
|---|---|---|
| glmS | 1.39 | Glucosamine-6-phosphate synthase |
| Genes missing in the microarray | ||
| addA | ATP-dependent DNase | |
| comEC | Late competence operon required for DNA binding and uptake | |
| comP | Two-component sensor histidine kinase involved in early competence | |
| Genes with unchanged expression | ||
| comA | 0.84 | Two-component response regulator of late competence genes and surfactin production |
| comX | 1.00 | Competence pheromone precursor |
| comQ | 1.16 | Transcriptional regulator of late competence operon and surfactin expression |
| comS | 0.71 | Assembly link between regulatory components of the competence pathway |
| srfAA | 0.85 | Surfactin synthetase/competence |
| pnpA | 0.85 | Polynucleotide phosphorylase (PNPase) |
| ylbF | 0.73 | Unknown; positively controls ComK at a posttranscriptional level |
| mecA | 1.27 | Negative regulator of competence |
| clpX | 1.13 | ATP-dependent Clp protease |
| clpC | 0.84 | Class III stress response-related ATPase |
| clpP | 0.80 | ATP-dependent Clp protease proteolytic subunit |
| ypbH | 1.18 | Unknown; similar to negative regulator of competence; MecA homologue |
| codY | 0.88 | Transcriptional pleiotropic repressor |
| degU | 0.92 | Two-component response regulator involved in degradative enzyme and competence |
| abrB | 0.81 | Transcriptional pleiotropic regulator of transition state genes |
| rapC | 1.01 | Response regulator, aspartate phosphatase |
| addB | 0.97 | ATP-dependent DNase |
| rok | 1.29 | Repressor of comK |
| recA | 0.67 | Multifunctional protein involved in homologous recombination and DNA repair |
| spx | 1.70 | Negative effector of competence |
| spo0A | 1.35 | Two-component response regulator central for the initiation of sporulation |
| Genes repressed at least twofold in the yvcJ mutant strain | ||
| comK | 0.42 | Competence transcription factor |
| comGA | 0.23 | Late competence gene |
| comGB | 0.24 | DNA transport machinery |
| comGC | 0.27 | Exogenous DNA binding |
| comGD | 0.26 | DNA transport machinery |
| comGE | 0.27 | DNA transport machinery |
| comGF | 0.29 | DNA transport machinery |
| comGG | 0.29 | DNA transport machinery |
| comER | 0.29 | Nonessential gene for competence |
| comEA | 0.20 | Exogenous DNA-binding protein |
| comEB | 0.39 | Nonessential gene for competence |
| comFA | 0.21 | Late competence protein required for DNA uptake |
| comFB | 0.26 | Late competence gene |
| comFC | 0.28 | Late competence gene |
| ywpH | 0.26 | Unknown; similar to single-strand DNA-binding protein |
| nin | 0.34 | Inhibitor of the DNA-degrading activity of NucA |
| comC | 0.41 | Late competence protein |
| yvyF | 0.36 | Unknown; similar to flagellar protein |
| nucA | 0.33 | Membrane-associated nuclease |