TABLE 3.
ComS overexpression bypasses yvcJ deletion for competence and gene expressiona
| Strain | Relevant genotype | Transformation frequency (106) | Relative gene expression determined by q-RT-PCR
|
||
|---|---|---|---|---|---|
| comS | comK | comGA | |||
| 168 | Wild type | 46.0 ± 7.1 | 1 | 1 | 1 |
| SG91 | yvcJ | 6.6 ± 1.6 | 0.7 ± 0.3 | 0.60 ± 0.18 | 0.60 ± 0.21 |
| SG121 | pcomS | 64.5 ± 10.6 | 3.1 ± 0.4 | 1.06 ± 0.03 | 1.27 ± 0.06 |
| SG122 | yvcJ pcomS | 31.5 ± 7.8 | 4.9 ± 1.8 | 1.60 ± 0.39 | 1.65 ± 0.44 |
a
Cells of the wild-type strain and of yvcJ mutant strains in which ComS was overexpressed were transformed with 250 ng of a chromosomal DNA containing a spectinomycin cassette. Transformation frequencies were determined as described in Materials and Methods. The relative expression of the comS, comK, and comGA genes in the wild-type strain and in mutant strains in which ComS was overexpressed was determined by q-RT-PCR. All the values are averages of data from at least three independent experiments.