TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype and/or phenotypea | Source |
|---|---|---|
| Strains | ||
| E. coli Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| C. burnetii Nine Mile, phase II, clone 4 (RSA439) | ΔCBU0678-CBU0698, truncated lipopolysaccharide | 17a |
| Plasmids | ||
| pCR2.1-Topo | Kmr Ampr pUC ori f1 ori lacZα | Invitrogen |
| pCR-BLUNTII-Topo | Kmr Zeor pUC ori lacZα ccdB | Invitrogen |
| pBVS2Himar1C9 | pBVS2 containing the Himar1C9 transposase; Kmr ColE1 ori | P. Stewart (unpublished) |
| pITR-flgB-GENT | Gmr ColE1 ori | P. Stewart (unpublished) |
| pcDNA6.2-C-mCherry-DEST | Ampr Cmr pUC ori f1 ori ccdB mCherry | S. Grieshaber (unpublished) |
| pENTR-D-Topo | Kmr pUC ori ccdB | Invitrogen |
| pEXP1-DEST | Ampr Cmr pUC ori ccdB | Invitrogen |
| pDEST-15 | Ampr Cmr pBR322 ori GST ccdB | Invitrogen |
| pCR2.1-Himar1C9 | Himar1C9 transposase gene cloned into pCR2.1-Topo; Kmr Ampr pUC ori f1 ori lacZα | This study |
| pCR2.1-P1169 | CBU1169 promoter cloned into pCR2.1-Topo; Kmr Ampr pUC ori f1 ori lacZα | This study |
| pCR2.1-P1169-Himar1C9 | CBU1169 promoter from pCR2.1-P1169 cloned into pCR2.1-Himar1C9; Kmr Ampr pUC ori f1 ori lacZα | This study |
| pITR-flgB-GENT-BB | pITR-flgB-GENT containing additional BspHI and BamHI restriction sites; Gmr ColE1 ori | This study |
| pBLUNTII-P1169 | CBU1169 promoter cloned into pCR-BLUNTII-Topo; Kmr Zeor pUC ori lacZα ccdB | This study |
| pBLUNTII-CAT | CAT gene cloned into pCR-BLUNTII-Topo; Cmr Kmr Zeor pUC ori lacZα ccdB | This study |
| pBLUNTII-P1169-CAT | CAT gene from pBLUNTII-CAT cloned into pBLUNTII-P1169; Kmr Zeor pUC ori lacZα ccdB | This study |
| pITR-P1169-CAT | P1169-CAT fragment cloned into pITR-flgB-GENT; Cmr ColE1 ori | This study |
| pCR2.1-mCherry | mCherry gene cloned into pCR2.1-Topo; Kmr Ampr pUC ori f1 ori lacZα | This study |
| pTn | mCherry gene from pCR2.1-mCherry cloned into pITR-P1169-CAT; Cmr ColE1 ori | This study |
| pBLUNTII-1898 | CBU1898 gene cloned into pCR-BLUNTII-Topo; Kmr Zeor pUC ori lacZα ccdB | This study |
| p1898-Tn | CBU1898 gene cloned into pTn; Cmr ColE1 ori | This study |
| pENTR-D-ftsZ | ftsZ gene cloned into pENTR-D-Topo, Kmr pUC ori | This study |
| pENTR-D-ftsZ::Tn | ftsZ::Tn gene cloned into pENTR-D-Topo, Kmr pUC ori | This study |
| pENTR-D-ftsA | ftsA gene cloned into pENTR-D-Topo, Kmr pUC ori | This study |
| pEXP1-ftsZ | ftsZ gene cloned into pEXP1-DEST via LR clonase II reaction, Ampr pUC ori | This study |
| pEXP1-ftsZ::Tn | ftsZ::Tn gene cloned into pEXP1-DEST via LR clonase II reaction, Ampr pUC ori | This study |
| pDEST-15-ftsA | ftsA gene cloned into pDEST-15 via LR clonase II reaction, Ampr pBR322 ori | This study |
a
Zeor, zeocin resistance; Tetr, tetracycline resistance; Cmr, Cm resistance; Ampr, ampicillin resistance; Strr, streptomycin resistance; Gmr, gentamicin resistance; Kmr, Km resistance.