TABLE 2.
TaqMan qPCR, PCR and sequencing oligonucleotides
| Primer or probe | Sequence (5′ to 3′)a |
|---|---|
| PCR and sequencing | |
| P1169-PstI-F | GCGCTGCAGAAACAGGTTCTCTAATTAATCCGG |
| P1169-NdeI-R | GCGCATATGCGCTCTCCTTTCAGAAGGATT |
| C9Himar1-NdeI-F | GCGCATATGGAAAAAAAGGAATTTCGTGTTTTG |
| C9Himar1-HindIII-R | GCGAAGCTTTTATTCAACATAGTTCCCTTCAAG |
| pITR-flgB-GENT-BamHI-F | CGCGGATCCAGCCGAGATCGGCTTACGCG |
| pITR-flgB-GENT-BspHI-R | CGCTCATGAGTTAGGTGGCGGTACTTGGG |
| P1169 AvaIF | GCGCCCGAGAAACAGGTTCTCTAATTAATCCGG |
| CAT-NdeI-F | CGCCATATGGAGAAAAAAATCACTGGATATACC |
| CAT-EcoRV-R | CGCGATATCTTACGCCCCGCCCTGC |
| mCherry-BspHI-F | CGCTCATGATCGTGAGCAAGGGCGAGGAG |
| mCherry-BamHI-R | CGCGGATCCTTACTTGTACAGCTCGTCCATGC |
| CBU1898-NotI-F | CCCGCGGCCGCATGGACCCACCCCTTAAAGACG |
| CBU1898-NotI-R | CCCGCGGCCGCTTAGGCGCCACAGCGATACG |
| ColE1-3′out | TTGTGATGCTCGTCAGGGG |
| CBU0141-F | CACCATGTTTGAACTGGGTGAAACCTC |
| CBU0141-R | TCAATCTTCCTCCAGGCGACG |
| CBU0141-B2c-R | TTACCGGGTTGGACTCAAGACGATAG |
| CBU0140-F | CACCATGGCGGCTATGCCGAAAAATCCAG |
| CBU0140-R | TCAAAAATTTCCTTGGAACCAACTTTTCATCC |
| qPCR | |
| CAT-taq-F | TTATACGCAAGGCGACAAGGT |
| CAT-taq-R | CCATCACAGACGGCATGATG |
| CAT-taq-Pb | CTGATGCCGCTGGCGATTCAGG |
| mCherry-taq-F | AACCGCCAAGCTGAAGGTTA |
| mCherry-taq-R | GGACAGTATGTCCCAGGCAAA |
| mCherry-taq-Pb | CAAAGGCGGGCCCCTGCC |
a
Underlined regions denote restriction site sequences.
b
Probes (indicated by ″-P″) have a 5′ 6-FAM dye and 3′ TAMRA quencher covalently linked to the oligonucleotide sequence.