TABLE 3.
Location of Tn insertions in C. burnetii transformants
| Electroporation expt | Gene disrupteda | No. of digest rescue clones
|
Protein size (aa) | Predicted effect on protein sequence | Protein function | |
|---|---|---|---|---|---|---|
| ClaI | PsiI | |||||
| 1 | CBU0573 | 0 | 1 | 715 | 17 aa from Tn added after aa 522 | Acyl-CoA dehydrogenase |
| CBU0964 | 1 | 0 | 303 | Truncation at aa 275 | Hypothetical protein | |
| CBU1196 | 0 | 1 | 753 | 10 aa from Tn added after aa 444 | ATP-dependent Clp protease | |
| CBU1745 | 1 | 1 | 230 | 10 aa from Tn added after aa 70 | Membrane endopeptidase M50 | |
| CBU2020 | 0 | 5 | 476 | 17 aa from Tn added after aa 351 | Glutamate/gamma-aminobutyrate antiporter | |
| 2 | CBU0806 | 0 | 2 | 35 | 17 aa from Tn added after aa 22 | Hypothetical protein |
| CBU2021* | 2 | 9 | 431 | Truncation at aa 106 | Hypothetical ATPase | |
| CBU2032* | 1 | 0 | 254 | 17 aa from Tn added after aa 131 | GGDEF family protein | |
| Intergenic between CBU2078 and CBU2079 | 0 | 2 | Tn insertion between CBU2079 promoter and RBS, expression no longer off native promoter | |||
| 3 | CBU0736 | 2 | 0 | 217 | 12 aa from Tn added after aa 92 | Hypothetical exported protein |
| CBU1418 | 1 | 0 | 157 | 17 aa from Tn added after aa 46 | Putative regulatory protein | |
| 4 | CBU0003 | 1 | 0 | 357 | 17 aa from Tn added after aa 217 | DNA replication and repair protein RecF |
| CBU0036 | 2 | 0 | 159 | 12 aa from Tn added after aa 2 | (3R)-Hydroxymyristoyl-[acyl carrier protein] dehydratase, FabZ | |
| CBU0432* | 10 | 0 | 428 | Truncation at aa 258 | Transporter, MFS superfamily | |
| CBU0921 | 2 | 2 | 105 | 12 aa from Tn added after aa 77 | Hypothetical cytosolic protein | |
| CBUA0008c*† | 0 | 3 | 709 | Truncation at aa 446 | RHS family protein | |
| Intergenic between CBU1745 and CBU1746 | 0 | 1 | No effect? | - | ||
| 5 | CBU0062* | 0 | 1 | 501 | Truncation at aa 105 | DnaJ domain protein |
| CBU0535 | 0 | 1 | 323 | No RBS present (inserts in start codon, no change to coding) | Hypothetical protein | |
| CBU0713 | 0 | 2 | 416 | 10 aa from Tn added after aa 289 | Phosphate transporter | |
| CBU0804 | 0 | 2 | 1012 | 125 aa from Tn added after aa 985 | Acriflavin resistance plasma membrane protein | |
| CBU1048 | 0 | 2 | 280 | 10 aa from Tn added after aa 266 | Hypothetical membrane associated protein | |
| CBU1094 | 0 | 1 | 380 | 12 aa from Tn added after aa 135 | Acriflavin resistance periplasmic protein | |
| CBU1268 | 0 | 2 | 357 | 12 aa from Tn added after aa 239 | Hypothetical protein | |
| CBU1387 | 0 | 2 | 216 | 10 aa from Tn added after aa 92 | Hypothetical cytosolic protein | |
| CBU1430 | 5 | 1 | 309 | Truncation at aa 231 | tRNA pseudouridine synthase B | |
| CBU1701 | 1 | 2 | 468 | 17 aa from Tn added after aa 102 | Hypothetical membrane spanning protein | |
| CBU1711 | 1 | 0 | 62 | Inserts in stop codon, no effect on protein coding | Hypothetical protein | |
| Intergenic between CBU0113 and CBU0114 | 5 | 3 | Tn insertion between CBU0114 RBS and start codon, expression no longer off native promoter | |||
| Intergenic between CBU0031 and CBU0032 | 0 | 3 | Possible disruption of CBU0031 and CBU0032 promoters | |||
| 6 | Intergenic between CBU2048 and CBU2049 | 3 | 2 | Possible disruption of CBU2048 promoter | ||
| 7a | CBU1173 | 0 | 1 | 122 | Truncation at aa 20 | Hypothetical protein |
| 7a | CBU1410 | 1 | 0 | 430 | Truncation at aa 39 | Citrate synthase |
| 7b | CBUA0008d*† | 0 | 3 | 348 | 10 aa from Tn added after aa 180 | RHS family protein |
a
*, Pseudogene in one or more C. burnetii isolates; †, QpH1 plasmid gene.