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. 2008 Dec 29;191(5):1414–1428. doi: 10.1128/JB.01446-08

FIG. 10.

FIG. 10.

The protonophore CCCP negatively affects in vitro T3S and bacterial growth of X. campestris pv. vesicatoria. (A) In vitro T3S of HrpF is inhibited by a final CCCP concentration of 1 μM. X. campestris pv. vesicatoria strain 85* was incubated in secretion medium in the absence or presence of CCCP at final concentrations ranging from 0.5 to 5 μM as indicated. Total cell extracts (TE) and culture supernatants (SN) were analyzed by immunoblotting using an HrpF-specific antibody. (B) CCCP affects bacterial growth in minimal medium. X. campestris pv. vesicatoria strain 85* was incubated in minimal medium at pH 7.0 in the presence or absence of 1 μM CCCP as indicated, and the optical density of the cultures was measured over 8 hours. (C) CCCP inhibits bacterial multiplication under secretion-permissive conditions. X. campestris pv. vesicatoria strain 85* was incubated in minimal medium at pH 5.3 as described for panel B, and the optical density was measured over 8 hours.