TABLE 1.

Published bacterial strains and plasmids used in this study

Strain or plasmid	Relevant characteristicsa	Reference(s) or source
X. campestris pv. vesicatoria strains
85-10	Pepper race 2; wild type; Rifr	17, 39
85*	85-10 derivative containing the hrpG* mutation	69
85E*ΔhrcU	hrcU deletion mutant derivative of strain 85E*	66
85*ΔhpaC	hpaC deletion mutant derivative of strain 85*	14
82-8	Pepper race 1; wild type; Rifr	17, 39
82*	82-8 derivative containing the hrpG* mutation	69
E. coli strains
BL21(DE3)	F−ompT hsdSB (rB− mB−) gal dcm (DE3)	Stratagene, Heidelberg, Germany
DH5α	F−recA hsdR17(rK− mK+) φ80dlacZΔM15	Bethesda Research Laboratories, Bethesda, Md.
DH5α λpir	F−recA hsdR17(rK− mK+) φ80dlacZΔM15 [λpir]	44
Plasmids
pBluescript(II) KS	Phagemid, pUC derivative; Apr	Stratagene
pUC119	ColE1 replicon; Apr	63
pC3003	pUC19 containing a triple c-myc tag; Apr	Kindly provided by J. Kämper; unpublished
pRK2013	ColE1 replicon, TraRK+ Mob+; Kmr	30
pOK1	Suicide vector; sacB sacQ mobRK2 oriR6K; Smr	35
pGEX-2TKM	GST expression vector; ptac GST lacIq pBR322 ori; Apr, derivative of pGEX-2TK with polylinker of pDSK604	GE Healthcare, Munich, Germany (25)
pGhpaB	pGEX-2TKM expressing GST-HpaB	14
pGhpaC	pGEX-2TKM expressing GST-HpaC	14
pDSK602	Broad-host-range vector; contains triple lacUV5 promoter; Smr	25
pDSK604	Derivative of pDSK602 with modified polylinker	25
pDMhpaB	pDSK604 derivative encoding HpaB-c-Myc	13
pDMhpaC	pDSK604 derivative encoding HpaC-c-Myc	14
pLAFR6	RK2 replicon, Mob+ Tra−; multicloning site flanked by transcription terminators; Tcr	11
pL6xopC356	pLAFR6 derivative encoding XopC1-200-AvrBs3Δ2 under control of the native promoter	51
pL6xopJ356	pLAFR6 derivative encoding XopJ1-155-AvrBs3Δ2 under control of the native promoter	51

^aAp, ampicillin; Km, kanamycin; Rif, rifampin; Sm, spectinomycin; Tc, tetracycline.