TABLE 1.
E. coli strains
| Straina | Relevant genotype | Source or reference |
|---|---|---|
| AB1157 | Wild typeb | 4 |
| BW13635 | proC677(Tetr)::Tn5-132 | M. Berlyn |
| JC5519 | recB21 recC22 | 4 |
| JJC260 | sbcD300::kan | B. Michel |
| JJC979 | recF332::Tn3 | B. Michel |
| MG1655 | proAB+ | 4 |
| N2691 | recA269::Tn10 | R.G. Lloyd |
| STL113 | recJ2052::Tn10kan | 39 |
| STL2694 | ΔxonA300::cat | 39 |
| LMM1112 | recF332::Tn3 | P1.JJC979 × AB1157 to Apr UVs |
| LMM1123 | recB21 recC22 recF332::Tn3 | P1.JJC979 × JC5519 to Apr UVs |
| LMM1245 | recA269::Tn10 | P1.N2691 × AB1157 to Tcr UVs |
| LMM1246 | ΔxonA300::cat | P1.STL2694 × AB1157 to Cmr |
| LMM1247 | sbcD300::kan | P1.JJC260 × AB1157 to Kmr |
| LMM1248 | ΔxonA300::cat sbcD300::kan | P1.JJC260 × LMM1246 to Kmr |
| LMM1249 | ΔxonA300::cat sbcD300::kan recA269::Tn10 | P1.N2691 × LMM1248 to Tcr UVs |
| LMM1250 | ΔxonA300::cat recA269::Tn10 | P1.N2691 × LMM1246 to Tcr UVs |
| LMM1251 | sbcD300::kan recA269::Tn10 | P1.N2691 × LMM1247 to Tcr UVs |
| LMM1254 | recB21 recC22 recA269::Tn10 | P1.N2691 × JC5519 to Tcr UVs |
| LMM1292 | recB21 recC22 sbcD300::kan | P1.JJC260 × JC5519 to Kmr |
| LMM1315 | ΔxonA300::cat sbcD300::kan recF332::Tn3 | P1.JJC979 × LMM1248 to Apr UVs |
| LMM1317 | recB21 recC22 sbcD300::kan ΔxonA300::cat | P1.STL2694 × LMM1292 to Cmr UVr |
| LMM1318 | recB21 recC22 sbcD300::kan ΔxonA300::cat recA269::Tn10 | P1.N2691 × LMM1317 to Tcr UVs |
| LMM1326 | recB21 recC22 sbcD300::kan ΔxonA300::cat recF332::Tn3 | P1.JJC979 × LMM1317 to Apr UVs |
| LMM1651 | recJ2052::Tn10kan | P1.STL113 × AB1157 to Kmr |
| LMM1652 | recJ2052::Tn10kan recA269::Tn10 | P1.N2691 × LMM1651 to Tcr Uvs |
| LMM1638 | proAB+ | P1.MG1655 × AB1157 to Pro+ |
| LMM1639 | proAB+ proC677(Tetr)::Tn5-132 | P1.BW13635 × LMM1638 to Tcr Pro− |
| LMM1646 | proAB+ proC677(Tetr)::Tn5-132 recJ2052::Tn10kan | P1.STL113 × LMM1639 to Kmr |
| LMM2040 | proAB+recJ2052::Tn10kan proC+ sbcD300::kan | P1.LMM1247 × LMM1646 to Pro+ λpals |
| LMM2041 | proAB+ recJ2052::Tn10kan proC+ sbcD300::kan recA269::Tn10 | P1.N2691 × LMM2040 to Tcr UVs |
a
All strains except BW13635, MG1655, STL113, and STL2694 are derivatives of AB1157.
b
Markers are F− thr-1 ara-14 leuB6 Δ(gpt-proA)62 lacY1 tsx-33 supE44 galK2 λ− rac hisG4 rfbD1 mgl-51 rpsL31 kdgK51 xyl-5 mtl-1 argE3 thi-1 qsr′.