TABLE 2.
Production of anucleate cells in E. coli strains
| Strain | Relevant genotype | Total no. of cells counted | Anucleate cells (%)a |
|---|---|---|---|
| AB1157 | Wild type | 2,405 | 0 |
| LMM1245 | recA | 2,188 | 5.2 |
| LMM1246 | xonA | 1,866 | 0 |
| LMM1250 | xonA recA | 2,293 | 1.7 |
| LMM1247 | sbcD | 1,975 | 0 |
| LMM1251 | sbcD recA | 2,272 | 1.4 |
| LMM1248 | xonA sbcD | 2,020 | 0 |
| LMM1249 | xonA sbcD recA | 2,921 | 0.1 |
| JC5519 | recBC | 1,542 | 0 |
| LMM1254 | recBC recA | 2,890 | 0.07 |
| LMM1651 | recJ | 1,752 | 0 |
| LMM1652 | recJ recA | 2,560 | 4.3 |
| LMM2040 | recJ sbcD | 1,830 | 0 |
| LMM2041 | recJ sbcD recA | 2,551 | 1.4 |
a
Only cells showing no trace of DAPI fluorescence were considered to be anucleate.