TABLE 1.
Oligonucleotide | Sequence |
---|---|
q-RT-PCR | |
groESL_fw | CGATGGCTACGGTGTGAAAT |
groESL_rev | AGTGTCGTGCGCGGATTA |
rpoA_fw | CCGAGGTTGAGATTGATGGT |
rpoA_rev | CCTTTCAGGTTGAGCAGGAT |
HEX_groESL | 5′-HEX-TCCGAAAGCGACATTCTGGCAATT-3′-BHQ-1 |
FAM_rpoA | 5′-FAM-ACAGCACCAAAGAAGGCGTTCAGG-3′-BHQ-1 |
Northern blotting | |
groEL_probe_fw | CCATCTCCGCTAACTCCGAC |
groEL_probe_rev | GCAACCACGCCTTCTTCTAC |
dnaK_probe_fw | GACGAACCCGCAAAACAC |
dnaK_probe_rev | TGACCACCAACGAGGATAAC |
hslU_probe_fw | AACAGTGTGTAAACGACGAG |
hslU_probe_rev | GGAAGAGATGACCAGCCAG |
rpoH_probe_fw | CCCAGTTATCATCTTCAATGCC |
rpoH_probe_rev | GCAGCTAAAACGCTGATCC |
Cloning | |
dicF_ecor1_fw | TAGAATTCGCCTGTCGTTAAATTTTCGTC |
dicF_hind3_rev | TAAAGCTTACACCAATTTCAAAACAACCTTC |