TABLE 1.
Bacterial strains used in this study
| Strain | Genotype | Reference or source |
|---|---|---|
| AD16 | VH1000 with a single-copy derivative of pAD40 (carrying the complete promoter region P2hemA P1hemA and all of the hemA gene to nt +42 of the prfA1gene fused to lacZ) | 9 |
| BW25113 | rrnB3 ΔlacZ4787 hsdR514 ΔaraBAD567 ΔrhaBAD568 rph-1 | 4 |
| GRB1776 | MG1655 but ttcA1 zdx2425::mini-Tn10cam | G. Björk |
| IIG1 | AD16 but yciS::mini-Tn10 | This study |
| IIG2 | AD16 but cysB::IS10 yciS::mini-Tn10 | This study |
| IIG16 | JWK1267 but prfA1 zcg174::Tn10 | This study |
| IIG28 | GRB1776 but prfA1 zcg-174::Tn10 | This study |
| IIG29 | MRA8 but mnmA2 zcg-174::Tn10 | This study |
| IIG44 | JW0413 but prfA1 zcg-174::Tn10 | This study |
| IIG46 | JW0658 but prfA1 zcg-174::Tn10 | This study |
| JW0413 | BW25113 but ΔthiI | 4 |
| JW0658 | BW25113 but ΔmiaB | 4 |
| JW1267 | recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1/pCa24N(cysB) | 16 |
| JW3305 | BW25113 but ΔtusB | 4 |
| JWK1267 | BW25113 but ΔcysB | 4 |
| MG1655 | ilvG rfb-50 rph-1 | www.genome.wisc.edu; 5 |
| MRA7 | MG1655 but prfA1 zcg-174::Tn10 | M.R.-A.a |
| MRA8 | MG1655 but prfA1 | 34 |
| MRA475 | MG1655 but prfA1 ilv-3164::Tn10kan cysB::IS10 | M.R.-A. |
| MRA514 | MRA8 but mnmE ilv-135::Tn10 | M.R.-A. |
| MRA532 | MRA475 but yciS::mini-Tn10 | M.R.-A. |
| MRA533 | MRA475 but yciS::mini-Tn10 cysB+ | This work |
| TH178 | MG1655 fadR::Tn10 mnmA2 | T. Hagervall |
| VH1000 | MG1655 lacIZ Δ(Mlu) rph+ | 9 |
a
M.R.-A., M. Rydén-Aulin (strain collection).