TABLE 2.
Oligonucleotide primers used for PCR
| Gene or gene regiona | Upstream primer | Sequence (5′ to 3′) | Downstream primer | Sequence (5′ to 3′) |
|---|---|---|---|---|
| 5′ region of yspP | yspP-FF-BamHI | TCGGATCCCACCAGTTTTTTGGTCTGCGGA | yspP-FR-BglII | GGAGATCTTTTTTGTAAGCATTAGTCTAAC |
| 3′ region of yspP | yspP-RF-BglII | TAAGATCTCTGTACCTAAACCTATTTACGT | yspP-RR-XbaI | ATTCTAGATTGGGGTTAGTGATCGATTATT |
| 5′ region of sycP | sycP-FF-BamHI | CCGGATCCTAGCTATGCCACATCAACATTA | sycP-FR-BglII | CAAGATCTTCACAAATTTGCTGATAAATAT |
| 3′ region of sycP | sycP-RF-BglII | AAAGATCTTATTACAGCGTAGTTTTTGAAT | sycP-RR-XbaI | CATCTAGACCATTTCTGAGCTGGAACGCAT |
| 5′ region of yspP-sycP | yspP-FF-BamHI | TCGGATCCCACCAGTTTTTTGGTCTGCGGA | yspP-sycP-FR | CGCTGTAATAAGACGCATTAGTCTAACTCA |
| 3′ region of yspP-sycP | yspP-sycP-RF | CGTCTTATTACAGCGTAGTTTTTGA | sycP-RR-XbaI | CATCTAGACCATTTCTGAGCTGGAACGCAT |
| 5′ region of yopB | yopB-FF-BamHI | ACGGATCCGTTTCATTCAGAAATATGATTC | yopB-FR | ATCATGGGTTATCAACGCACTCATG |
| 3′ region of yopB | yopB-RF | TTGATAACCCATGATTAAGTTTAAGGAGGA | yopB-RR-XbaI | TGTCTAGAATTTGTTCCTGTTTAACTATTT |
| yspP | yspP-F | TGTGTAAGCTATCAATTCGGCGTT | yspP-R | TTCACAAATTTGCTGATAAATATCC |
| yspP-sycP | yspP-F | TGTGTAAGCTATCAATTCGGCGTT | sycP-R | TCAAAAACTACGCTGTAATAAGACG |
| yspP-sycP-his6 | yspP-F | TGTGTAAGCTATCAATTCGGCGTT | sycP-his6-R | CTAGTGGTGGTGGTGGTGGTGCGCTGTAATAAGACGATTATCCCATTCCTC |
| sycP | sycP-F | CGGATGAGAAACTTAATTTATCTGT | sycP-R | TCAAAAACTACGCTGTAATAAGACG |
| lacZ (1) | lacZ (1)-SmaI | AAACCCGGGAGGAAACAGCTATGCAGCAATCGCATCAGGCTGGTTACGCAAAC | cyaA (406)-EcoRI | TCGAATTCTTAGCTGTCATAGCCGGAA |
| yspP (1) | yspP-F | TGTGTAAGCTATCAATTCGGCGTT | yspP (1)-SmaI | TTTCCCGGGCATTAGTCTAACTCATTTAATG |
| yspP (1-21) | yspP-F | TGTGTAAGCTATCAATTCGGCGTT | yspP (21)-EcoRV | TTGATATCTAAAACAGATGTAACTATTTT |
| yspP (1-73) | yspP-F | TGTGTAAGCTATCAATTCGGCGTT | yspP (73)-EcoRV | GGGATATCTGCACTTTGTCCTGAAGTTAAT |
| yspP (1-124) | yspP-F | TGTGTAAGCTATCAATTCGGCGTT | yspP (124)-EcoRV | AAGATATCCCATAAAATATCCGTTTCGCTA |
| sycP-his6 | sycP-NdeI | GACATATGCTTACAAAAAACATCGCCC | sycP-XhoI | ATCTCGAGTTTTTTTATTATATTAACGTAA |
a
Numbers in parentheses indicate the specific amino acids encoded in the gene regions.