TABLE 1.

List of plasmids used or constructed in this study

Plasmid	Relevant phenotype	Antibiotic resistance markera	Source or reference
Vectors
pAM401	E. coli-E. faecalis shuttle vector	Cm and Tc	41
pDL278	E. coli-E. faecalis shuttle vector	Sp	27
pGEMTEasy	E. coli TA cloning vector	Ap	Promega
pHM13	E. coli-B. subtilis shuttle vector	Km	Unpublished (gift from H. Putzer, Institut de Biologie Physico-Chimique, France)
pYH56	E. coli-B. subtilis shuttle vector	Km (gram positive) and Ap (gram negative)	4
pYHII	HindIII-PstI fragment from pDAK608 containing RNA II cloned into pYH56	Km (gram positive) and Ap (gram negative)	This work
p043lacZ	Vector carrying lacZ gene	Cm	26
RNA I clones/mutants
pDAK607	pAM401 containing par	Cm	39
pDAK608	RNA II gene in pSPORT1	Ap	39
pDAK611	pDL278 containing RNA II	Sp	37
pDAK617	SalI-XbaI fragment of pDAK608 containing RNA II cloned into pAM401	Cm	This work
pDAK704	pAM401 containing wild-type RNA I gene	Cm	38
pDAK734	pAM401 with 19-stop RNA I construct A under native promoter	Cm	39
pDAK749*	pAM401 with RNA I construct B under native promoter	Cm	This work
pDAK773	pAM401 with RNA I construct C under native promoter	Cm	This work
pDAK762	pAM401 with RNA I construct D under native promoter	Cm	This work
pDAK763	pAM401 with RNA I construct E under native promoter	Cm	This work
pDAK770	pAM401 with RNA I construct F under native promoter	Cm	This work
pDAK771	pAM401 with RNA I construct G under native promoter	Cm	This work
T7 pDAK749*	pGEMTeasy with 19-stop RNA I construct B under T7 promoter	Ap	This work
T7 pDAK773	pGEMTeasy with RNA I construct C under T7 promoter	Ap	This work
T7 pDAK771	pGEMTeasy with RNA I construct G under T7 promoter	Ap	This work
pDAK787	pHM13 with RNA I construct A	Cm	This work
pDAK788	pHM13 with RNA I construct B	Cm	This work
pDAK789	pAM401 with RNA I promoter	Cm	This work
pDAK790	p043 carrying the lacZ gene expression under control of RNA I promoter	Cm	This work

^aCm, chloramphenicol; Tc, tetracycline; Sp, spectinomycin; Ap, ampicillin; Km, kanamycin.