TABLE 2.
Plasmids used in this study
| Designation | Characteristic(s) | Reference or source |
|---|---|---|
| pEVP3 | Integrative lacZ vector | 1 |
| pEVPblpMNO | Derivative of pEVP3 with the blpMNPO promoter cloned upstream of lacZ | This study |
| pE626 | pCR2.1 containing htrA with a serine-to-alanine mutation at the active site | 24 |
| pE668 | pCRII containing the bgaA gene with an internal deletion and replacement with the erm cassette | 12 |
| pBlpAL | pUC19 derivative with a P376 fragment containing blpMNPO | 2 |
| pBLPALΔN | In-frame deletion of blpN with an NsiI site | 2 |
| pBlpALNFLAG | C-terminal Flag epitope added to the blpN sequence | This study |
| pBlpALMFLAG | pBlpAL derivative with a Flag epitope introduced in-frame into the BstBI site of blpM | This study |
| pEVPhtrA | Derivative of pEVP3 with the htrA promoter cloned upstream of lacZ | This study |
| pBlpALblpA::erm | Derivative of pBlpAL with the erm cassette disrupting the open reading frame of blpA | This study |