FIG. 2.

pceA transcript level detected by RT-PCR upon subcultivation in the absence of PCE. Total RNA (1 μg) isolated from S. multivorans grown on pyruvate/fumarate-containing medium for 1, 15, 30, and 40 subcultivations (10% inoculum) was used as a template. To each lane an identical volume of 10 μl of the samples was applied to a 1% agarose gel. The gel was stained with ethidium bromide. −, without RT; +, with RT.