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Induction of PceA formation and PCE dechlorination by PceA-depleted S. multivorans grown on formate plus PCE (a and b) or TCE (c and d). PceA was detected by Western blot analysis (1 μg protein/lane) performed with PceA-specific antibodies. Sampling times are given above each lane. PCE, TCE, and cDCE were determined by gas chromatography in the aqueous phase (see Materials and Methods), and the total amount of these compounds was calculated. ▪, PCE; ⧫ TCE; ▴, cDCE, ○, growth (OD578).
