TABLE 1.

Primers

Analysis	Primer	Sequence	Locationa
Confirming that pUT1951 and	686	CTCCCGTATCGTAGTTATC	Internal to Ampr gene
pUT1945 are inserted into the chromosome (Fig. 3)	733	ACATACCCGACACATTG	c bp 1306725-1306741 Erwinia chromosome
Identifying plasmids formed	357	GGAAATGTTGAATACTCATACTCTTC	Internal to Ampr gene
in donors when replication proteins are supplied	718	GCGGCCGCAATTGAGCAGAGGTTCGCAGCC	c bp 1309304-1309320 Pectobacterium chromosome
Mapping Kanr DNA after transfer from JW2128 and	791	CGCCAATCGGCACAAACAATAG	c bp 2228862-2228883 MG1655 chromosome
JW2161	792	CAGTCATAGCCGAATAGCCT	Internal to Kanr gene, JW2128 and JW2161
	804	GCAGGCTGGATGCGTTAC	c bp 2265473-2265490 MG1655 chromosome
Cloning cryptic E. coli oriT	787	GGCCGGATCGATCGGCAACGCGACCGGT	c bp 2225942-2225957 MG1655 chromosome
	789	GGCCGGCAATTGAGCAGCTCAACGATGTCGCG	c bp 2226029-2226048 MG1655 chromosome
Replacement of cryptic oriT in E. coli with Camr FRT	795	CTTGCCACTTTCGGCAACGCGACCGGTTTAAGCTTCAATGACTTTGTCGTGTAGGCTGGAGCTGCTTC	Amplification of Camr DNA (reverse)
fragment	796	AGCTCAACGATGTCGCGGGGAAAATGAAAATTATTGAGGCACGGTTAAATGGGAATTAGCCATGGTCC	Amplification of Camr DNA (forward)

^ac, complement to the indicated base pairs. Amp^r, ampicillin resistance.