FIG. 3.
PypB and PypC interact with different regions of the hreP promoter. Y. enterocolitica carrying pBAD-pypB, pBAD-pypC, or the control plasmid pBAD18Kan and the β-galactosidase reporter plasmid pHreP-125, pHreP-250, pHreP-375, or pHreP-500, containing 125 nt, 250 nt, 373 nt, or 500 nt, respectively, 5′ of the hreP start codon transcriptionally fused to the lacZ gene, were grown in the presence of 0.2% arabinose to induce gene expression from the PBAD promoter for 3 h at 26°C before determination of β-galactosidase activity (in arbitrary Miller units). Data are means and standard deviations from at least three experiments, each performed in triplicate.