TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference | |
|---|---|---|---|
| Y. enterocolitica strains | |||
| JB580v | ΔyenR (r− m+) Nalr; serogroup O:8 | 21 | |
| GHY19 | JB580v hreP-lacZYA | 9 | |
| GHY271 | JB580v/pKW1-hreP (hreP-lacZYA) | This study | |
| GHY306 | JB580v pypA-lacZYA | This study | |
| GHY307 | JB580v pypB-lacZYA | This study | |
| GHY320 | JB580v ΔpypA | This study | |
| GHY329 | JB580v ΔpypB | This study | |
| GHY334 | JB580v pypC-lacZYA | This study | |
| GHY350 | JB580v ΔpypC | This study | |
| GHY351 | JB580v ΔpypA hreP-lacZYA | This study | |
| GHY352 | JB580v ΔpypB hreP-lacZYA | This study | |
| GHY353 | JB580v ΔpypC hreP-lacZYA | This study | |
| GHY366 | JB580v ΔpypB pypC-lacZYA | This study | |
| GHY372 | JB580v ΔpypA ΔpypB | This study | |
| GHY373 | JB580v ΔpypA ΔpypC | This study | |
| GHY374 | GHY372 hreP-lacZYA | This study | |
| GHY375 | GHY373 hreP-lacZYA | This study | |
| GHY376 | JB580v ΔpypB ΔpypC | This study | |
| GHY388 | GHY376 hreP-lacZYA | This study | |
| E. coli strains | |||
| DH5α | φ80dΔ(lacZ)M15 Δ(argF-lac)U169 endA1 recA1 hsdR17(rK− mK+) deoR thi-1 supE44 gyrA96 relA1 | Gibco BRL | |
| BL21(DE3) | B F−dcm ompT hsdS(rB− mB+) gal λ(DE3) | Novagen | |
| S17-1λpir | Tpr SmrrecA thi pro HsdR−M+RP4::2-Tc::Mu::Km Tn7λpir lysogen | 29 | |
| MC4100 | F− Δ(argF-lac)U169 rpsL150 deoC1 relA1ptsF25 flbB5501 rbsR | 14 | |
| PD145 | MC4100 (hns205::Tn10) | 14 | |
| Plasmids | |||
| pEP185.2 | Camrmob+ (RP4) R6K ori (suicide vector) | 21 | |
| pEP-pypAΔ | Camr; pypA deletion construct | This study | |
| pEP-pypBΔ | Camr; pypB deletion construct | This study | |
| pEP-pypCΔ | Camr; pypC deletion construct | This study | |
| pFUSE | Camrmob+ (RP4) R6K ori (suicide vector) lacZYA | 1 | |
| pFUSE-pypA | Camr, pypA promoter fragment in pFUSE | This study | |
| pFUSE-pypB | Camr, pypB promoter fragment in pFUSE | This study | |
| pKN8 | Camrmob+ (RP4) R6K ori (suicide vector) lacZYA pFUSE with additional BglII site | 33 | |
| pKN8-pypC | Camr; pypC promoter fragment in pKN8 | This study | |
| pET24b(+) | Kanr; T7 promoter expression vector | Novagen | |
| pET-pypBΔc | pypB missing 99 nt from the 3′ end in pET24b(+) | This study | |
| pET-pypC | pypC in pET24b(+) | This study | |
| pBAD18Kan | Kanr; PBAD expression vector | 11 | |
| pBAD-pypA | pypA in pBAD18Kan | This study | |
| pBAD-pypB | pypB in pBAD18Kan | This study | |
| pBAD-pypC | pypC in pBAD18Kan | This study | |
| pBAD-rovA | rovA in pBAD18Kan | This study | |
| pAJD428 | TnMod-RKm′-lacIqtacp delivery plasmid | 27 | |
| pWKS30 | Ampr pSC101 ori; low-copy-number cloning vector | 43 | |
| pWKS30-lac | lacZYA of pFUSE in pWKS30 | This study | |
| pSmUC | Ampr Smr/Spr; Smr/Spr resistance cassette in pUC129 | 31 | |
| pKW1 | Ampr Smr/Spr; Smr/Spr resistance cassette of pSmUC in pWKS30-lac | This study | |
| pKW1-hreP | Ampr Smr/Spr; hreP-lacZYA reporter plasmid | This study | |
| pHreP-125 | Ampr Smr/Spr; pKW1-based hreP-lacZYA reporter plasmid including 125 nt 5′ of the hreP start codon | This study | |
| pHreP-250 | Ampr Smr/Spr; pKW1-based hreP-lacZYA reporter plasmid including 250 nt 5′ of the hreP start codon | This study | |
| pHreP-375 | Ampr Smr/Spr; pKW1-based hreP-lacZYA reporter plasmid including 373 nt 5′ of the hreP start codon | This study | |
| pHreP-500 | Ampr Smr/Spr; pKW1-based hreP-lacZYA reporter plasmid including ca. 500 nt 5′ of the hreP start codon | This study | |