TABLE 2.
Primers used in this study
| Oligonucleotide | Sequence (5′→3′)a | Restriction site |
|---|---|---|
| JS-rhpA1 | GGAATTCCACCATTTTAAGTTTAATGATATT | EcoRI |
| JS-rhpA2 | GGGGTACCTTATCGCTGCTGCTCCATCGACAT | KpnI |
| JS-rhpB1 | GCTCTAGAGATCTTAAAGCAATCAAAATAAAAATAAAT | XbaI |
| JS-rhpB2 | AACTGC AGTTAAGGATTTGGTTTCTCCCCACT | PstI |
| JS-rhpC1 | GGGGTACCCATCTATAGTGTGATTTATTTTTA | KpnI |
| JS-rhpC2 | GCTCTAGATTAATAGCCGGTAAATCTATCTGT | XbaI |
| JS-pypA1 | CTCAGGGCCAGTAATGGGGAA | |
| JS-pypA2 | TATTAGTACCAGCACGTAGCG | |
| JS-pypB1 | GCTCTAGAGCCGTTGCATCACTAAGACTG | XbaI |
| JS-pypB2 | CTGTTCTTTCTCTGTTACTCC | |
| JS-pypB3 | CTAGCTAGCGAGACAAATGAGATTGTGTTTAAA | NheI |
| JS-pypB4 | CCGCTCGAGCTTTTTCCAGCGATAGCTTTGCAG | XhoI |
| JS-pypC1 | GCTCTAGAGAATGCATGACTCACCGCTT | XbaI |
| JS-pypC2 | GAAGATCTCAAACACATTACCAATCTCTT | BglII |
| JS-pypC3 | CTAGCT AGCTATGTTGATACAGTCTCTGTTGTT | NheI |
| JS-pypC4 | CCGCTCGAGATAGCCGGTAAATCTATCTGTCAT | XhoI |
| JShreP124f | GCTCTAGACTGTTGAGGAAATAGGTAGATTGA | XbaI |
| JShreP250f | GCTCTAGATTGAGTTGGAATGGATTGATGAGT | XbaI |
| JShreP373f | GCTCTAGAAGGCGTTCCCATCTAAGAGAAAAT | XbaI |
| JShreP-200r | GGAATTCTTGACGACATAGGTGTTGGCACTG | EcoRI |
| flhBX.rev | GCTCTAGATGGAAAAACACATCGAAGCCG | XbaI |
| flhB2Xrev | GCTCTAGAATCTGGCCTTTCTCGCGAGCCTTC | XbaI |
| hrePB.rev | CGGGATCCCTTGTGCTGAGGTAAAATAAG | BamHI |
| hreP2.rev | CGGAATTCTATCATAAGTAACGTCAAATCGTT | EcoRI |
| Tacp.out | TCGGCTCGTATAATGTGTGG | |
| tnp.for | TACCGATTTATCCGCAATCCC | |
| tnp.rev | CTTGTCCAGATAGCCCAGTAG | |
| KW-pypA1.for | GGGGTACCCCAGATAGGAATTGAGTGGTG | KpnI |
| KW-pypA2.rev | TCCCCGCGGACCGCATTGATGTGGATAAGG | SacII |
| KW-pypA1.rev | CCGCTCGAGGGACTGAGTAAGCAGCGAAAT | XhoI |
| KW-pypA2.for | CCGCTCGAGTGCCGGTCTGGTTGCTCCAAG | XhoI |
| KW-pypB1.for | GGGGTACCCTGATACAGTCTACTCCAACC | KpnI |
| KW-pypB2.rev | GCTCTAGATTGCTAATAGTCCGATAACGA | XbaI |
| KW-pypB1.rev | CCGCTCGAGGGCAGCTACCTATCCTGTATA | XhoI |
| KW-pypB2.for | CCGCTCGAGACAACCTGGAACACGTTTAATAGC | XhoI |
| KW-pypC1.for | GGGGTACCGAATGCATTGACTCACCGCTT | KpnI |
| KW-pypC2.rev | GCTCTAGAGGTAACGTAGAATACGTAACT | XbaI |
| KW-pypC1.rev | CCGCTCGAGCCCCTACAAATATATTCACTC | XhoI |
| KW-pypC2.for | CCGCTCGAGATCAGATAGTCATAACACCCT | XhoI |
| KR-cpxA1 | CCGCTCGAGATGCTGGAGCAACACATTGAG | XhoI |
| GH-cpx9 | GAAGATCTGCCCGATAAAGTTACGCACCAT | BglII |
| rovA3 | CGGAATTCTGGTAGTTATGCTAGCACGCTA | EcoRI |
| rovA4 | GCTCTAGATTACTTACTTTGTAGTTGAATA | XbaI |
a
Restriction sites are underlined.