TABLE 3.
Transcription of hreP in pyp deletion strainsa
| Strain | β-Galactosidase activity (fold increase over activity of control) under the following condition:
|
|||
|---|---|---|---|---|
| Control | PypAOP | PypBOP | PypCOP | |
| GHY19 (wild type) | 56.4 ± 8.1 | 401.8 ± 135.2 (7) | 383 ± 38.1 (6.8) | 304.5 ± 30 (5.4) |
| GHY351 (ΔpypA) | 30.4 ± 1.9 | 232.3 ± 75.8 (8) | 337.9 ± 42.3 (11) | 67.1 ± 18.8 (2) |
| GHY352 (ΔpypB) | 46.2 ± 17.2 | 320.2 ± 1.3 (7) | 229.3 ± 38.9 (5) | 307.7 ± 37.8 (7) |
| GHY353 (ΔpypC) | 31.1 ± 0.2 | 183.4 ± 10.4 (6) | 275.2 ± 41.8 (9) | 180.3 ± 60.8 (6) |
| GHY374 (ΔpypA ΔpypB) | 22.8 ± 6.4 | 269.7 ± 47.3 (12) | 115.1 ± 23.8 (5) | 58 ± 11.8 (3) |
| GHY375 (ΔpypA ΔpypC) | 23.7 ± 2.9 | 233.9 ± 32.9 (10) | 360.9 ± 29.2 (15) | 90.9 ± 21.3 (4) |
| GHY388 (ΔpypB ΔpypC) | 65.7 ± 20.3 | 281 ± 28.7 (4) | 219.1 ± 32.1 (3) | 144.2 ± 30.9 (2) |
a
Shown are β-galactosidase activities (in Miller units) of hreP-lacZ fusion strains with deletions of pyp genes after overproduction (PypOP) of PypA, PypB, or PypC by growing bacteria in the presence of 0.2% arabinose for 3 h. Bacteria carrying the empty pBAD18Kan plasmid served as a control. Data are means ± standard deviations for at least three individual experiments, each performed in triplicate.