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. 2008 Dec 12;191(5):1581–1586. doi: 10.1128/JB.01588-08

FIG. 2.

FIG. 2.

Gel mobility shift assay of various DNA fragments with His-RpaB. DIG-labeled upstream DNA fragments of hliB (−69 to +51), psaAB (+80 to +179), psaK1 (−90 to +63), and psaLI (−90 to +54) were incubated for 30 min with His-RpaB added at the indicated concentrations. Samples were separated on a 6% polyacrylamide gel. For the competition assay of psaK1, unlabeled probe fragment (−90 to +63: self) or nonspecific fragment from the coding region (+61 to +213: non-self) was added at a 150-fold excess of the probe concentration. For the competition assay of psaLI, unlabeled probe fragment (−90 to +54: self) or nonspecific fragment from the coding region (+121 to +264: non-self) was added at a 200-fold excess of the probe concentration.