TABLE 2.
Expression of genes in the gts operon of R. leguminosarum mutant strains relative to Rlv3841 as measured by qRT-PCR
| Straina (genotype) | Mean fold expression ± SEM relative to Rlv3841b
|
||||
|---|---|---|---|---|---|
| gtsR | pRL100242 | gtsA | gtsB | gabD4 | |
| RU1722 (aapJQM braEF) | 0.7 ± 0.6 | 1.3 ± 0.3 | 2.1 ± 0.1 | 0.9 ± 0.2 | 1.6 ± 0.2 |
| RU1736 (aapJQM braEF gtsR101) | 4.4 ± 0.1 | 61.9 ± 0.1 | 80.4 ± 0.2 | 33.8 ± 0.2 | 16.2 ± 0.2 |
| RU2228 (aapJQM braEF gtsR101, pRL100244::Tn5) | 3.0 ± 0.1 | 75.7 ± 0.1 | 4.8 ± 0.1 | 2.2 ± 0.1 | 1.1 ± 0.4 |
a
Cells were grown overnight in 10 mM glucose-10 mM ammonia AMS.
b
qRT-PCR was performed on three independent cultures.