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. 2008 Dec 29;191(5):1490–1497. doi: 10.1128/JB.01469-08

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Copyright © 2009, American Society for Microbiology

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FIG. 3. — M. maripaludis MMP0704 constructs can complement an S. enterica apbC mutant. Strains were grown at 37°C in no-carbon essential salts medium supplemented with thiamine, with tricarballylate as the sole carbon and energy source. (A) The growth of strain DM10300 with pJMB100 (apbC) (open squares), pJMB105 (Δ1-19 MMP0704) (open circles), pJMB109 [MMP0704(Cys5,9,12,18Ala)] (filled triangles), pJMB104 (MMP0704) (filled circles), and empty vector (pSU18) (filled squares) was determined by measuring the OD₆₅₀. (B) Mutational analysis of conserved cysteine residues in the Δ1-19 MMP0704 gene product. The growth of strain DM10300 with pJMB105 (Δ1-19 MMP0704) (open circles), pJMB111 [Δ1-19 MMP0704(Cys218Ala)] (closed squares), pJMB114 [Δ1-19 MMP0704(Cys220Ala)] (open squares), and pJMB115 [Δ1-19 MMP0704(Cys218,220Ala)] (closed circles) was monitored as described in the legend to panel A.