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. 2008 Dec 29;191(5):1490–1497. doi: 10.1128/JB.01469-08

FIG. 4.

FIG. 4.

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of an overloaded, Coomassie blue-stained gel shows the purities and solubilities of heterologously expressed MMP0704 and MJ0283 proteins (10 μg each). Marker proteins, shown in lane M, have the molecular masses indicated to the left. Lane 1 contains affinity-purified MMP0704-His6 protein, with an apparent molecular mass of 33 kDa (calculated mass, 32.1 kDa). Lane 2 contains affinity-purified MMP0704(20-289)-His6 protein, with an apparent molecular mass of 29 kDa (calculated mass, 30.4 kDa). Lane 3 contains heat-stable, purified MJ0283 protein, with an apparent molecular mass of 31 kDa (calculated mass, 31.2 kDa). Lane 4 contains heat-stable, purified MJ0283(19-290) protein, with an apparent molecular mass of 30 kDa (calculated mass, 29.5 kDa).