TABLE 1.
List of microorganisms and plasmids
| Strain or plasmid (parent plasmid) | Description and/or partial genotype | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| BL21(DE3) | Expression strain with T7 RNA polymerase gene | Novagen |
| DH5α | General cloning host | Invitrogen |
| M. jannaschii JAL-1 | Wild type | DSM 2261 |
| M. maripaludis S900 | S2 Δhpt | 25 |
| S. enterica DM10300 | ara-9 apbC55::Tn10d(tet) | 4 |
| S. solfataricus P2 | Wild type | DSM 1617 |
| Plasmids | ||
| pDG499 (pET-11a) | MJ0283 (wild type) | This work |
| pDG530 (pET-11a) | Δ1-18 MJ0283; encodes MJ0283(19-290) protein | This work |
| pDG547 (pET-20b) | Δ1-19 MMP0704; encodes MMP0704(20-289)-His6 protein | This work |
| pDG549 (pET-20b) | MMP0704 (wild type); encodes MMP0704-His6 protein | This work |
| pDG592 (pET-20b) | MMP0704(Cys5,9,12,18Ala) | This work |
| pET-11a | Expression vector | Novagen |
| pET-20b | Expression vector | Novagen |
| pJMB100 (pSU18) | S. enterica apbC (wild type) | This work |
| pJMB102 (pSU18) | MJ0283 (wild type) | This work |
| pJMB103 (pSU18) | Δ1-18 MJ0283 | This work |
| pJMB104 (pSU18) | MMP0704 (wild type) | This work |
| pJMB105 (pSU18) | Δ1-19 MMP0704 | This work |
| pJMB107 (pSU18) | MMP0704(Ser55Ala) | This work |
| pJMB108 (pSU18) | MMP0704(Cys218Ala) | This work |
| pJMB109 (pSU18) | MMP0704(Cys5,9,12,18Ala) | This work |
| pJMB110 (pSU18) | Δ1-19 MMP0704(Ser55Ala) | This work |
| pJMB111 (pSU18) | Δ1-19 MMP0704(Cys218Ala) | This work |
| pJMB112 (pSU18) | MMP0704(Cys220Ala) | This work |
| pJMB113 (pSU18) | MMP0704(Cys218,220Ala) | This work |
| pJMB114 (pSU18) | Δ1-19 MMP0704(Cys220Ala) | This work |
| pJMB115 (pSU18) | Δ1-19 MMP0704(Cys218,220Ala) | This work |
| pJMB116 (pSU18) | SSO0460 | This work |
| pSU18 | Complementation vector | 2 |