TABLE 2.
Oligodeoxyribonucleotide primers
| Primer name | Sequencea |
|---|---|
| 5MJ0283BN | CGAGGATCCCATATGGCTGAGTGTGATGGAAAATG |
| 3MJ0283B | GCAGGATCCTTATTCTTTTTTACCTTCGACC |
| 5MJ0283BN2 | CGCCATATGGATACAAAGAAACTCTTAGC |
| 5MMP0704N | GCAGCATATGGCAGAAGAATGCTCTGGAAATTG |
| 3MMP0704X | GCAGTTACTCGAGTTCTTTTTTAATTCTTTCAAGAACTGTATTAAC |
| 5MMP0704N2 | GCAGCATATGGATACTAAAAAAATGATGGAACAGCAGAACG |
| 5MMP0704c | ATGGCAGAAGAAGCCTCTGGAAATGCCGATAGCGCCGGTTCAAG |
| 5MMP0704e | GCAGCATATGGCAGAAGAAGCC |
| 3MMP0704d | CATCATTTTTTTAGTATCTGAAGCGTCGGAACTTGAACCGGCGCTA |
| C218A | TGTTGAAAACATGGGCGGATTTGTAGCTCCTGAATGCGACAAA |
| C218A-rev | TTTGTCGCATTCAGGAGCTACAAATCCGCCCATGTTTTCAACA |
| S55A | GGTAAAGGAGGAGTTGGTAAAGCCACAGTTACCG |
| S55A-rev | CGGTAACTGTGGCTTTACCAACTCCTCCTTTACC |
| C220A | AACATGGGCGGATTTGTATGTCCTGAAGCCGACAAACTAATTG |
| C220A-rev | CAATTACTTTGTCGGCTTCAGGACATACAAATCCGCCCATGTT |
| C218AC220A | AACATGGGCGGATTTGTAGCTCCTGAAGCCGACAAAGTAATTTG |
| C218AC220A rev | CAATTACTTTGTCGGCTTCAAGGAGCTACAAATCCGCCCATGTT |
| 5SSO0460N | GCAGCATATGAGCAGTAATCCTTTTAG |
| 3SSO0460B | GCAGGATCCTTATTGATTGGATTCAACAATTT |
| T7-Promoter | GTAATACGACTCACTATAGGG |
| T7-Term | GCTAGTTATTGCTCAGCGG |
| −40 FORWARD | GTTTTCCCAGTCACGAC |
| −48 REVERSE | GCGGATAACAATTTCACACAGGA |
a
Restriction sites are underlined, and the initiator codon is shown in boldface.