TABLE 3.
Archaeal ApbC/Npb35 homologs can function in place of S. enterica ApbC in vivoa
| Vector | Vector insert | Doubling timeb (h) with:
|
|
|---|---|---|---|
| Glucose | Tricarballylate | ||
| pJMB100 | apbC | 1.1 ± 0.0 | 1.6 ± 0.1 |
| pJMB102 | MJ0283 | 1.1 ± 0.0 | NGc |
| pJMB103 | Δ1-18 MJ0283 | 1.1 ± 0.0 | 3.1 ± 0.2 |
| pJMB104 | MMP0704 | 1.1 ± 0.1 | 3.0 ± 0.3 |
| pJMB107 | MMP0704(Ser55Ala) | 1.2 ± 0.0 | NG |
| pJMB108 | MMP0704(Cys218Ala) | 1.1 ± 0.1 | NG |
| pJMB112 | MMP0704(Cys220Ala) | 1.1 ± 0.0 | NG |
| pJMB114 | MMP0704(Cys218,220Ala) | 1.0 ± 0.1 | NG |
| pJMB109 | MMPO704(Cys5,9,12,18Ala) | 1.1 ± 0.0 | 2.7 ± 0.3 |
| pJMB105 | Δ1-19 MMP0704 | 1.1 ± 0.0 | 2.2 ± 0.0 |
| pJMB110 | Δ1-19 MMP0704(Ser55Ala) | 1.1 ± 0.0 | NG |
| pJMB111 | Δ1-19 MMP0704(Cys218Ala) | 1.1 ± 0.0 | 2.2 ± 0.0 |
| pJMB114 | Δ1-19 MMP0704(Cys220Ala) | 1.1 ± 0.0 | 2.6 ± 0.1 |
| pJMB115 | Δ1-19 MMP0704(Cys218,220Ala) | 1.1 ± 0.0 | NG |
| pJMB116 | SSO0460 | 1.2 ± 0.0 | 2.7 ± 0.1 |
| pJMB106 | None | 1.1 ± 0.0 | NG |
a
Complementation was conducted as outlined in Materials and Methods.
b
Doubling times were calculated using the following formulas: μ = ln(X/Xo)/T, where μ is the growth rate, X is the OD650 value at a given time point, X0 is the OD650 value at time zero, and T is the time between readings X and Xo, and doubling time (g) = (ln 2)/μ (26). The numbers shown represent the averages of results for three independent cultures. Minimal media with the indicated carbon sources were used. The media were supplemented with thiamine.
c
NG, no growth.