FIG. 3.

Mapping and sequence analysis of the L. plantarum ftsH promoter. (A) Primer extension analysis of ftsH mRNA. Total RNA was isolated from L. plantarum WCFS1 cells grown to exponential phase at 30°C (control [C]) and after incubation at either 30°C or 42°C for 15, 30, or 60 min. Lanes G, A, T, and C show DNA sequencing products obtained on genomic DNA with the same primer used for primer extension. The corresponding nucleotide sequence is shown on the left. The transcription start site is indicated by an asterisk. (B) Nucleotide sequence of the ftsH promoter region. −10 and −35 sequences and the transcriptional start site are in boldface. The ctsR binding site is underlined. The translation initiation codon (ATG) is in boldface.