TABLE 1.
Oligonucleotides used in this study
| Oligonucleotidea | Sequence (5′-3′) | Application |
|---|---|---|
| FtsHP | CGTTTGTGAGCCGGAATTACCGC | Primer extension analyses |
| ldhDF | ACGCCCAAGCTGATGTTATATC | qRT-PCR |
| ldhDR | AGTGTCCCACGAGCAAAGTT | qRT-PCR |
| gyrAF | CATGCGGTTAGGCGATGAT | qRT-PCR |
| gyrAR | ATCGGCGTTGACGGTTTG | qRT-PCR |
| FtsH1F | TTACGGTCTTGACTATGCAGAACGCTATCGGAACT | Gel mobility shift assay |
| FtsH2F | TCGATGAAGGAGGCACATATGAACAATCGACGCA | Gel mobility shift assay |
| FtsH1R | GCTGGATACTAACGTTTTTAACGTTATTCTTATCTAATTG | Gel mobility shift assay |
| FB1ctsR | ATCTCGAGTTAAAATCCTGCGGTTAGTG | ctsR chromosomal deletion |
| RB1ctsR* | GACTTTGCATGTGCTTCACC | ctsR chromosomal deletion |
| FB2ctsR* | CGGGCCCGGATAATTATCGG | ctsR chromosomal deletion |
| RB2ctsR* | TCATCCGTAATCGTAACCCG | ctsR chromosomal deletion |
| FBctsR* | TGAACCGGCAACAAGGCATG | ctsR chromosomal deletion |
| CtsRrtF | AATTTGGTCGATGATGCTGATG | qRT-PCR |
| CtsRrtR | TAAGTCCCGGTCCGTTAATCC | qRT-PCR |
| CtsR1 | GGTGGTCTCCCATGCAAAGTCAAAATATC | ctsR overexpression |
| CtsR2 | CTCCTCGAGGCTTTCGTAACGCAAGTGGTT | ctsR overexpression |
| CatF | TCAAATACAGCTTTTAGAACTGG | ctsR chromosomal deletion |
| CatR | CCAGTAAATGAAGTCCATGGA | ctsR chromosomal deletion |
| ftsHKOF | ATGGTACCGGACTTATTCGAACAAGCTAAG | ftsH disruption |
| ftsHKOR | TAGGATCCGTAAGCTGCTTGTTGGTTG | ftsH disruption |
| pUCeryF | CCAGGCTTTACACTTTATGC | ftsH disruption |
| pUCeryF | TGGAAAGTTACACGTTACTAAAG | ftsH disruption |
| ftsHrtF | GCAGCTACCTTCGAAGAATCCA | qRT-PCR |
| ftsHrtR | GGGAAACTTGGTTCAGCAACA | qRT-PCR |
| ftsHF | AAAACTGCAGAATCGACGCAATGGAC | ftsH disruption |
| ftsHR | GCTCTAGACGCTCATAACCGAATTAACG | ftsH disruption |
a
Asterisks indicate 5′-phosphorylated oligonucleotides.