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. 2008 Dec 29;29(6):1649–1660. doi: 10.1128/MCB.01076-08

FIG. 5.

FIG. 5.

hSPT20/hSAGA is recruited to the promoter of ER stress-responsive genes after thapsigargin induction. (A) qPCR analysis of reverse transcribed RNA shows a significant activation of the four ER stress-responsive genes. mRNA levels were normalized to cyclophilin B and are presented as change compared to the control DMSO-treated sample. Samples were analyzed 1, 2, 3, and 4 h after treatment. (B to E) Time course ChIP results obtained with RNA Pol II (B), SPT20 (C), SPT3 (D), and ATXN7L3 (E) antibodies are shown as a percentage of input genomic DNA. Different shadings represent the promoter sequences of the corresponding ER stress-inducible genes analyzed by qPCR (indicated below the axis). The results obtained in five different conditions (nontreated sample; 1 h, 2 h, 3 h, and 4 h of thapsigargin treatment) are represented from left to right for each promoter. The promoter of GAPDH and a noncoding sequence serve as controls. Similar results were obtained in two biological replicates. Results obtained in a representative experiment are shown with the SD calculated from qPCR triplicates for each time point.