FIG. 3.

CA cluster in intron 6 binds hnRNP L, as well as hnRNP LL. Biotinylated 5′ and 3′ parts (lanes 2 and 3, respectively) of the CA cluster of hnRNP L intron 6, as well as biotinylated (CA)₃₂ RNA (lane 4) or an unrelated biotinylated control RNA (lane 5), were incubated in HeLa nuclear extract, followed by neutravidin-agarose selection, release of bound protein, and Western blot analysis with anti-hnRNP L monoclonal antibody 4D11 (top panel) or anti-hnRNP LL antibody (bottom panel). In a control reaction, no RNA was added (lane 6). For comparison, 10% of the nuclear extract input was analyzed (lane 1).