FIG. 3.
Loss of inhibitory phosphorylation of separase results in lethality of preimplantation embryos. (A) A litter of embryos at the blastocyst stage from a mutant superovulated female. Arrowheads indicate degenerating embryos. (B) Quantitation of blastocysts produced in control and mutant females. *, P < 0.05 (t test). (C) Analysis of developmental stages of in vitro-cultured embryos. *, P < 0.05; **, P < 0.001 (t test). (D) Micrographs of eight-cell embryos. Arrows indicate fragmented/degenerated cells. (E) Genotyping of embryos at different developmental stages.