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Recombinant human eIF4GI proteins. (A) Diagram of eIF4GI constructs. PABP-, eIF4E-, eIF4A-, and eIF3-binding sites and the RNA-binding region are indicated. (B) Purified murine eIF4E and human eIF4GI fragments (2 μg) were analyzed by SDS-12% PAGE followed by Coomassie blue staining. The amino acid numbers of eIF4GI fragments are indicated above the lanes. The positions of molecular mass markers (in kilodaltons) are indicated to the left of the gel.
