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. 2009 Jan 5;29(6):1421–1431. doi: 10.1128/MCB.01158-08

FIG. 1.

FIG. 1.

The proline-rich domain is required for Blimp-1-mediated gene repression and function. (A) Plasmids encoding full-length Blimp-1, proline-rich-domain-deleted Blimp-1 (Δ317-424), or empty vector alone (Control) together with a firefly luciferase reporter driven by CIITA promoter III (CIITA pIII-Luc) and a Renilla luciferase reporter plasmid (RL-tk) were cotransfected into 293T cells for 2 days and then measured for luciferase activity. (B) Plasmids encoding a full-length Blimp-1/Gal4 DNA binding domain (DBD), fusion protein (WT), proline-rich-domain-deleted Blimp-1/Gal4 DBD fusion protein (Δ317-424), or Gal4 DBD alone (DBD), together with (Gal4)4-tk-Luc and RL-tk, were transfected into 293T cells. Two days later, cell lysates were harvested for a luciferase activity assay. (A and B) Results are means ± standard deviations from three experiments. (C and D) Purified splenic Prdm1f/fCD19Cre+/+ (KO) and Prdm1f/fCD19+/+ (Control) B cells stimulated with LPS overnight were transduced with the indicated retroviral vectors. (C) ELISPOT results of the number of IgM-secreting cells from sorted YFP+ cells. (D) Flow cytometric analysis of surface expression of CD138 and B220 on YFP+ cells at day 3 of culture. Values shown indicate the percentages of B220lowCD138+ plasma cells. (C and D) Results are data from one representative experiment of three independent experiments.